Electron Microscopy:Article Title: A prophage-expressed type IV pilus component provides anti-phage defense.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse α-FLAG antibody (monoclonal) Sigma Cat# F3165; RRID: AB_259529 Goat α-mouse-AF555 Abcam Cat# AB150078; RRID:AB272519 Bacterial and virus strains P. aeruginosa PAO1 A. Davidson Lab GenBank: NC_002516.2 P. aeruginosa PAK A. Davidson Lab GenBank: LR657304.1 P. aeruginosa PAK (JBD68) A. Davidson Lab N/A P. aeruginosa PAK (LES2) A. Davidson Lab N/A P. aeruginosa PAK (LES3) A. Davidson Lab N/A P. aeruginosa PAK (JBD68 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAK (LES3 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAO1 ΔfimU A. Davidson Lab 16 N/A P. aeruginosa PAO1 ΔpilA A. Davidson Lab 42 N/A P. aeruginosa PAO1 ΔpilT A. Davidson Lab 42 N/A P. aeruginosa PAO1 I-C CRISPR A. Davidson Lab 43 N/A P. aeruginosa PAO1 I-C CRISPR Δhel A. Davidson Lab N/A P. aeruginosa PAO1 pilA A86C, pilT::Tn5 C. Ellison Lab 21 N/A E. coli DH5α A. Davidson Lab N/A E. coli SM10 λpir A. Davidson Lab N/A E. coli BTH101 A. Davidson Lab 32 N/A P. aeruginosa phage JBD68 A. Davidson Lab GenBank: KY707339.1 P. aeruginosa phage JBD68 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp4 A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp10 A. Davidson Lab N/A P. aeruginosa phage F10 A. Davidson Lab GenBank: NC_007805.1 P. aeruginosa phage LES2 A. Davidson Lab N/A P. aeruginosa phage LES3 A. Davidson Lab N/A P. aeruginosa phage LES3 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage DMS3 A. Davidson Lab GenBank: NC_008717.1 P. aeruginosa phage D3112 A. Davidson Lab GenBank: AY394005.1 P. aeruginosa phage M6 A. Davidson Lab GenBank: NC_007809.1 P. aeruginosa phage F116 A. Davidson Lab GenBank: NC_006552.1 P. aeruginosa phage Luz19 A. Davidson Lab GenBank: NC_010326.1 P. aeruginosa phage AB05 A. Davidson Lab GenBank: NC_026602.1 P. aeruginosa phage ΦKMV A. Davidson Lab GenBank: NC_005045.1 P. aeruginosa phage ΦKMZ A. Davidson Lab N/A Chemicals, peptides, and recombinant proteins Tris Bioshop Cat# TRS003 Sodium chloride Bioshop Cat# SOD004 BioTryptone BioBasic Cat# TG217 Magnesium sulfate (heptahydrate) BioBasic Cat# M5BO329 L-(+)Arabinose Bioshop Cat# ARB222.100 Phosphate buffered saline Bioshop Cat# PBS408 Tween 20 Bioshop Cat# TWN508 (Continued on next page) Cell Reports 45, 116759, January 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Agar Bioshop Cat# AGR100.500 Ultra Pure Agarose Invitrogen Cat# 16500_500 Difco MacConkey Agar Base BD Cat# 281810 Difco Maltose BD Cat# 216830 X-Gal Biobasic Cat# BB0083 Mitomycin C Cell Signaling Technologies Cat# 51854S NcoI-HF New England Biolabs Cat# R3193S EcoRI-HF New England Biolabs Cat# R3101S HindIII-HF New England Biolabs Cat# R3104S KpnI-HF New England Biolabs Cat# R3142S XbaI-HF New England Biolabs Cat# R0145S BsaI-HF v2 New England Biolabs Cat# R3733S T4 DNA Ligase New England Biolabs Cat# M0202S T4 Polynucleotide Kinase New England Biolabs Cat# M0201S T4 DNA Ligase Buffer New England Biolabs Cat# B0202A DpnI ThermoFisher Cat# ER1705 Uranyl acetate Electron Microscopy Sciences Cat# 22400 Gentamycin sulfate Bioshop Cat# GTA401 Ampicillin Bioshop Cat# AMP201 Kanamycin Bioshop Cat# KAN201 AF488-maleimide ThermoFisher Cat# A10254 Crystal violet Bioshop Cat# CRY422.25 Carbon-coated TEM grids Electron Microscopy Sciences Cat# CF150-Cu Deposited data PAO1 and PAO1 (JBD68) RNA-Seq raw reads This study SRA: PRJNA1364051 Recombinant DNA pHERD30T Qiu et al. 44 N/A pEX18Gm Hmelo et al. 45 N/A pKT25 Karimova et al. 46 N/A pUT18C Karimova et al. 46 N/A PAO1 FimU This study N/A PA14 FimU This study N/A JBD68 P-FimU This study N/A Les P-FimU This study N/A F10 P-FimU This study N/A BWH P-FimU This study N/A CAAD P-FimU This study N/A NSYG P-FimU This study N/A Zip Taylor et al. 9 N/A LES3 Repressor This study N/A JBD68 P-FimU 3xFLAG This study N/A PAO1 FimU 3xFLAG This study N/A pPTP4 JBD68 This study N/A pPTP4 LES2 This study N/A T25 JBD68 P-FimU This study N/A T25 F10 P-FimU This study N/A T25 PAO1 FimU This study N/A T25 PAO1 FimS This study N/A (Continued on next page) 14 Cell Reports 45, 116759, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER T18 PAO1 FimS This study N/A T18 PAO1 PilA This study N/A T18 PAO1 PilE Marko et al. 31 N/A T18 PAO1 PilX Marko et al. 31 N/A T18 PAO1 PilV Marko et al. 31 N/A T18 PAO1 PilW Marko et al. 31 N/A T18 PAO1 PilU Marko et al. 31 N/A T18 PAO1 PilB Marko et al. 31 N/A Oligonucleotides PAO1 fimU F: AAACCATGGCCTC ATATCGTTCCAACTCGACCGG Eurofins Genomics NcoI PAO1 fimU R: AAAAAGCTTTCAA TAGCATGACTGGGGCGC Eurofins Genomics HindIII PA14 fimU F: AAACCATGGCCC GCTCTATTTGTCGCAGCGCC Eurofins Genomics NcoI PA14 fimU R: AAAAAGCTTTCA GTTACAGCTGTCCGGTTGCTTTG Eurofins Genomics HindIII JBD68 p-fimU F: AAACCATGGCC TACTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI JBD68 p-fimU R: AAAAAGCTTTC AGCAGCCAGAAGCGTCCC Eurofins Genomics HindIII F10 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTA Eurofins Genomics NcoI F10 p-fimU R: AAAAAGCTTCTAAC AGTCAAAAGTATCCCAGGATTCC Eurofins Genomics HindIII LES3 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI LES3 p-fimU R: AAAAAGCTTTCAG CAGCCAGAAGTGTCCCA Eurofins Genomics HindIII BWH p-fimU F: AAACCATGGCC GGCTCCAAG Eurofins Genomics NcoI BWH p-fimU R: AAAAAGCTTCTAA CTGGGTTTTACAGCGCAGGc Eurofins Genomics HindIII CAAD p-fimU F: AAACCATGGGTT GAAATGTACTCTAGGTCGCGCG Eurofins Genomics NcoI CAAD p-fimU R: AAAAAGCTTTCAG GTTGGTTTCTTGGCGCA Eurofins Genomics HindIII NSYG p-fimU F: AAACCATGGATTC GCATAAATCAAGCTCAGCGTGG Eurofins Genomics NcoI NSYG p-fimU R: AAAAAGCTTCTAT TGGTCCTTGGCACACTGTG Eurofins Genomics HindIII LES3 repressor F: AAACCATGGCCA TCGATATCACGACAATCCGCCGCGCGAAC Eurofins Genomics NcoI LES3 repressor R: AAAAAGCTTTCAG TGTCGCCGCCGGCGCA Eurofins Genomics HindIII JBD68 p-fimU 3xFLAG F: TATAAAGAT CATGATATTGATTATAAAGATGATGAT GATAAATGAAAGCTTGGCACTGGCCG Eurofins Genomics Restriction free JBD68 p-fimU 3xFLAG R: ATCACCATC ATGATCTTTATAATCTCCACCACTTCC ACCTGCGCAGCCAGAAGCGTCCCAAG Eurofins Genomics Restriction free (Continued on next page) Cell Reports 45, 116759, January 27, 2026 15
Transmission Electron Microscopy:Article Title: A prophage-expressed type IV pilus component provides anti-phage defense.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse α-FLAG antibody (monoclonal) Sigma Cat# F3165; RRID: AB_259529 Goat α-mouse-AF555 Abcam Cat# AB150078; RRID:AB272519 Bacterial and virus strains P. aeruginosa PAO1 A. Davidson Lab GenBank: NC_002516.2 P. aeruginosa PAK A. Davidson Lab GenBank: LR657304.1 P. aeruginosa PAK (JBD68) A. Davidson Lab N/A P. aeruginosa PAK (LES2) A. Davidson Lab N/A P. aeruginosa PAK (LES3) A. Davidson Lab N/A P. aeruginosa PAK (JBD68 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAK (LES3 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAO1 ΔfimU A. Davidson Lab 16 N/A P. aeruginosa PAO1 ΔpilA A. Davidson Lab 42 N/A P. aeruginosa PAO1 ΔpilT A. Davidson Lab 42 N/A P. aeruginosa PAO1 I-C CRISPR A. Davidson Lab 43 N/A P. aeruginosa PAO1 I-C CRISPR Δhel A. Davidson Lab N/A P. aeruginosa PAO1 pilA A86C, pilT::Tn5 C. Ellison Lab 21 N/A E. coli DH5α A. Davidson Lab N/A E. coli SM10 λpir A. Davidson Lab N/A E. coli BTH101 A. Davidson Lab 32 N/A P. aeruginosa phage JBD68 A. Davidson Lab GenBank: KY707339.1 P. aeruginosa phage JBD68 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp4 A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp10 A. Davidson Lab N/A P. aeruginosa phage F10 A. Davidson Lab GenBank: NC_007805.1 P. aeruginosa phage LES2 A. Davidson Lab N/A P. aeruginosa phage LES3 A. Davidson Lab N/A P. aeruginosa phage LES3 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage DMS3 A. Davidson Lab GenBank: NC_008717.1 P. aeruginosa phage D3112 A. Davidson Lab GenBank: AY394005.1 P. aeruginosa phage M6 A. Davidson Lab GenBank: NC_007809.1 P. aeruginosa phage F116 A. Davidson Lab GenBank: NC_006552.1 P. aeruginosa phage Luz19 A. Davidson Lab GenBank: NC_010326.1 P. aeruginosa phage AB05 A. Davidson Lab GenBank: NC_026602.1 P. aeruginosa phage ΦKMV A. Davidson Lab GenBank: NC_005045.1 P. aeruginosa phage ΦKMZ A. Davidson Lab N/A Chemicals, peptides, and recombinant proteins Tris Bioshop Cat# TRS003 Sodium chloride Bioshop Cat# SOD004 BioTryptone BioBasic Cat# TG217 Magnesium sulfate (heptahydrate) BioBasic Cat# M5BO329 L-(+)Arabinose Bioshop Cat# ARB222.100 Phosphate buffered saline Bioshop Cat# PBS408 Tween 20 Bioshop Cat# TWN508 (Continued on next page) Cell Reports 45, 116759, January 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Agar Bioshop Cat# AGR100.500 Ultra Pure Agarose Invitrogen Cat# 16500_500 Difco MacConkey Agar Base BD Cat# 281810 Difco Maltose BD Cat# 216830 X-Gal Biobasic Cat# BB0083 Mitomycin C Cell Signaling Technologies Cat# 51854S NcoI-HF New England Biolabs Cat# R3193S EcoRI-HF New England Biolabs Cat# R3101S HindIII-HF New England Biolabs Cat# R3104S KpnI-HF New England Biolabs Cat# R3142S XbaI-HF New England Biolabs Cat# R0145S BsaI-HF v2 New England Biolabs Cat# R3733S T4 DNA Ligase New England Biolabs Cat# M0202S T4 Polynucleotide Kinase New England Biolabs Cat# M0201S T4 DNA Ligase Buffer New England Biolabs Cat# B0202A DpnI ThermoFisher Cat# ER1705 Uranyl acetate Electron Microscopy Sciences Cat# 22400 Gentamycin sulfate Bioshop Cat# GTA401 Ampicillin Bioshop Cat# AMP201 Kanamycin Bioshop Cat# KAN201 AF488-maleimide ThermoFisher Cat# A10254 Crystal violet Bioshop Cat# CRY422.25 Carbon-coated TEM grids Electron Microscopy Sciences Cat# CF150-Cu Deposited data PAO1 and PAO1 (JBD68) RNA-Seq raw reads This study SRA: PRJNA1364051 Recombinant DNA pHERD30T Qiu et al. 44 N/A pEX18Gm Hmelo et al. 45 N/A pKT25 Karimova et al. 46 N/A pUT18C Karimova et al. 46 N/A PAO1 FimU This study N/A PA14 FimU This study N/A JBD68 P-FimU This study N/A Les P-FimU This study N/A F10 P-FimU This study N/A BWH P-FimU This study N/A CAAD P-FimU This study N/A NSYG P-FimU This study N/A Zip Taylor et al. 9 N/A LES3 Repressor This study N/A JBD68 P-FimU 3xFLAG This study N/A PAO1 FimU 3xFLAG This study N/A pPTP4 JBD68 This study N/A pPTP4 LES2 This study N/A T25 JBD68 P-FimU This study N/A T25 F10 P-FimU This study N/A T25 PAO1 FimU This study N/A T25 PAO1 FimS This study N/A (Continued on next page) 14 Cell Reports 45, 116759, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER T18 PAO1 FimS This study N/A T18 PAO1 PilA This study N/A T18 PAO1 PilE Marko et al. 31 N/A T18 PAO1 PilX Marko et al. 31 N/A T18 PAO1 PilV Marko et al. 31 N/A T18 PAO1 PilW Marko et al. 31 N/A T18 PAO1 PilU Marko et al. 31 N/A T18 PAO1 PilB Marko et al. 31 N/A Oligonucleotides PAO1 fimU F: AAACCATGGCCTC ATATCGTTCCAACTCGACCGG Eurofins Genomics NcoI PAO1 fimU R: AAAAAGCTTTCAA TAGCATGACTGGGGCGC Eurofins Genomics HindIII PA14 fimU F: AAACCATGGCCC GCTCTATTTGTCGCAGCGCC Eurofins Genomics NcoI PA14 fimU R: AAAAAGCTTTCA GTTACAGCTGTCCGGTTGCTTTG Eurofins Genomics HindIII JBD68 p-fimU F: AAACCATGGCC TACTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI JBD68 p-fimU R: AAAAAGCTTTC AGCAGCCAGAAGCGTCCC Eurofins Genomics HindIII F10 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTA Eurofins Genomics NcoI F10 p-fimU R: AAAAAGCTTCTAAC AGTCAAAAGTATCCCAGGATTCC Eurofins Genomics HindIII LES3 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI LES3 p-fimU R: AAAAAGCTTTCAG CAGCCAGAAGTGTCCCA Eurofins Genomics HindIII BWH p-fimU F: AAACCATGGCC GGCTCCAAG Eurofins Genomics NcoI BWH p-fimU R: AAAAAGCTTCTAA CTGGGTTTTACAGCGCAGGc Eurofins Genomics HindIII CAAD p-fimU F: AAACCATGGGTT GAAATGTACTCTAGGTCGCGCG Eurofins Genomics NcoI CAAD p-fimU R: AAAAAGCTTTCAG GTTGGTTTCTTGGCGCA Eurofins Genomics HindIII NSYG p-fimU F: AAACCATGGATTC GCATAAATCAAGCTCAGCGTGG Eurofins Genomics NcoI NSYG p-fimU R: AAAAAGCTTCTAT TGGTCCTTGGCACACTGTG Eurofins Genomics HindIII LES3 repressor F: AAACCATGGCCA TCGATATCACGACAATCCGCCGCGCGAAC Eurofins Genomics NcoI LES3 repressor R: AAAAAGCTTTCAG TGTCGCCGCCGGCGCA Eurofins Genomics HindIII JBD68 p-fimU 3xFLAG F: TATAAAGAT CATGATATTGATTATAAAGATGATGAT GATAAATGAAAGCTTGGCACTGGCCG Eurofins Genomics Restriction free JBD68 p-fimU 3xFLAG R: ATCACCATC ATGATCTTTATAATCTCCACCACTTCC ACCTGCGCAGCCAGAAGCGTCCCAAG Eurofins Genomics Restriction free (Continued on next page) Cell Reports 45, 116759, January 27, 2026 15
RNA sequencing:Article Title: A prophage-expressed type IV pilus component provides anti-phage defense.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse α-FLAG antibody (monoclonal) Sigma Cat# F3165; RRID: AB_259529 Goat α-mouse-AF555 Abcam Cat# AB150078; RRID:AB272519 Bacterial and virus strains P. aeruginosa PAO1 A. Davidson Lab GenBank: NC_002516.2 P. aeruginosa PAK A. Davidson Lab GenBank: LR657304.1 P. aeruginosa PAK (JBD68) A. Davidson Lab N/A P. aeruginosa PAK (LES2) A. Davidson Lab N/A P. aeruginosa PAK (LES3) A. Davidson Lab N/A P. aeruginosa PAK (JBD68 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAK (LES3 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAO1 ΔfimU A. Davidson Lab 16 N/A P. aeruginosa PAO1 ΔpilA A. Davidson Lab 42 N/A P. aeruginosa PAO1 ΔpilT A. Davidson Lab 42 N/A P. aeruginosa PAO1 I-C CRISPR A. Davidson Lab 43 N/A P. aeruginosa PAO1 I-C CRISPR Δhel A. Davidson Lab N/A P. aeruginosa PAO1 pilA A86C, pilT::Tn5 C. Ellison Lab 21 N/A E. coli DH5α A. Davidson Lab N/A E. coli SM10 λpir A. Davidson Lab N/A E. coli BTH101 A. Davidson Lab 32 N/A P. aeruginosa phage JBD68 A. Davidson Lab GenBank: KY707339.1 P. aeruginosa phage JBD68 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp4 A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp10 A. Davidson Lab N/A P. aeruginosa phage F10 A. Davidson Lab GenBank: NC_007805.1 P. aeruginosa phage LES2 A. Davidson Lab N/A P. aeruginosa phage LES3 A. Davidson Lab N/A P. aeruginosa phage LES3 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage DMS3 A. Davidson Lab GenBank: NC_008717.1 P. aeruginosa phage D3112 A. Davidson Lab GenBank: AY394005.1 P. aeruginosa phage M6 A. Davidson Lab GenBank: NC_007809.1 P. aeruginosa phage F116 A. Davidson Lab GenBank: NC_006552.1 P. aeruginosa phage Luz19 A. Davidson Lab GenBank: NC_010326.1 P. aeruginosa phage AB05 A. Davidson Lab GenBank: NC_026602.1 P. aeruginosa phage ΦKMV A. Davidson Lab GenBank: NC_005045.1 P. aeruginosa phage ΦKMZ A. Davidson Lab N/A Chemicals, peptides, and recombinant proteins Tris Bioshop Cat# TRS003 Sodium chloride Bioshop Cat# SOD004 BioTryptone BioBasic Cat# TG217 Magnesium sulfate (heptahydrate) BioBasic Cat# M5BO329 L-(+)Arabinose Bioshop Cat# ARB222.100 Phosphate buffered saline Bioshop Cat# PBS408 Tween 20 Bioshop Cat# TWN508 (Continued on next page) Cell Reports 45, 116759, January 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Agar Bioshop Cat# AGR100.500 Ultra Pure Agarose Invitrogen Cat# 16500_500 Difco MacConkey Agar Base BD Cat# 281810 Difco Maltose BD Cat# 216830 X-Gal Biobasic Cat# BB0083 Mitomycin C Cell Signaling Technologies Cat# 51854S NcoI-HF New England Biolabs Cat# R3193S EcoRI-HF New England Biolabs Cat# R3101S HindIII-HF New England Biolabs Cat# R3104S KpnI-HF New England Biolabs Cat# R3142S XbaI-HF New England Biolabs Cat# R0145S BsaI-HF v2 New England Biolabs Cat# R3733S T4 DNA Ligase New England Biolabs Cat# M0202S T4 Polynucleotide Kinase New England Biolabs Cat# M0201S T4 DNA Ligase Buffer New England Biolabs Cat# B0202A DpnI ThermoFisher Cat# ER1705 Uranyl acetate Electron Microscopy Sciences Cat# 22400 Gentamycin sulfate Bioshop Cat# GTA401 Ampicillin Bioshop Cat# AMP201 Kanamycin Bioshop Cat# KAN201 AF488-maleimide ThermoFisher Cat# A10254 Crystal violet Bioshop Cat# CRY422.25 Carbon-coated TEM grids Electron Microscopy Sciences Cat# CF150-Cu Deposited data PAO1 and PAO1 (JBD68) RNA-Seq raw reads This study SRA: PRJNA1364051 Recombinant DNA pHERD30T Qiu et al. 44 N/A pEX18Gm Hmelo et al. 45 N/A pKT25 Karimova et al. 46 N/A pUT18C Karimova et al. 46 N/A PAO1 FimU This study N/A PA14 FimU This study N/A JBD68 P-FimU This study N/A Les P-FimU This study N/A F10 P-FimU This study N/A BWH P-FimU This study N/A CAAD P-FimU This study N/A NSYG P-FimU This study N/A Zip Taylor et al. 9 N/A LES3 Repressor This study N/A JBD68 P-FimU 3xFLAG This study N/A PAO1 FimU 3xFLAG This study N/A pPTP4 JBD68 This study N/A pPTP4 LES2 This study N/A T25 JBD68 P-FimU This study N/A T25 F10 P-FimU This study N/A T25 PAO1 FimU This study N/A T25 PAO1 FimS This study N/A (Continued on next page) 14 Cell Reports 45, 116759, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER T18 PAO1 FimS This study N/A T18 PAO1 PilA This study N/A T18 PAO1 PilE Marko et al. 31 N/A T18 PAO1 PilX Marko et al. 31 N/A T18 PAO1 PilV Marko et al. 31 N/A T18 PAO1 PilW Marko et al. 31 N/A T18 PAO1 PilU Marko et al. 31 N/A T18 PAO1 PilB Marko et al. 31 N/A Oligonucleotides PAO1 fimU F: AAACCATGGCCTC ATATCGTTCCAACTCGACCGG Eurofins Genomics NcoI PAO1 fimU R: AAAAAGCTTTCAA TAGCATGACTGGGGCGC Eurofins Genomics HindIII PA14 fimU F: AAACCATGGCCC GCTCTATTTGTCGCAGCGCC Eurofins Genomics NcoI PA14 fimU R: AAAAAGCTTTCA GTTACAGCTGTCCGGTTGCTTTG Eurofins Genomics HindIII JBD68 p-fimU F: AAACCATGGCC TACTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI JBD68 p-fimU R: AAAAAGCTTTC AGCAGCCAGAAGCGTCCC Eurofins Genomics HindIII F10 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTA Eurofins Genomics NcoI F10 p-fimU R: AAAAAGCTTCTAAC AGTCAAAAGTATCCCAGGATTCC Eurofins Genomics HindIII LES3 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI LES3 p-fimU R: AAAAAGCTTTCAG CAGCCAGAAGTGTCCCA Eurofins Genomics HindIII BWH p-fimU F: AAACCATGGCC GGCTCCAAG Eurofins Genomics NcoI BWH p-fimU R: AAAAAGCTTCTAA CTGGGTTTTACAGCGCAGGc Eurofins Genomics HindIII CAAD p-fimU F: AAACCATGGGTT GAAATGTACTCTAGGTCGCGCG Eurofins Genomics NcoI CAAD p-fimU R: AAAAAGCTTTCAG GTTGGTTTCTTGGCGCA Eurofins Genomics HindIII NSYG p-fimU F: AAACCATGGATTC GCATAAATCAAGCTCAGCGTGG Eurofins Genomics NcoI NSYG p-fimU R: AAAAAGCTTCTAT TGGTCCTTGGCACACTGTG Eurofins Genomics HindIII LES3 repressor F: AAACCATGGCCA TCGATATCACGACAATCCGCCGCGCGAAC Eurofins Genomics NcoI LES3 repressor R: AAAAAGCTTTCAG TGTCGCCGCCGGCGCA Eurofins Genomics HindIII JBD68 p-fimU 3xFLAG F: TATAAAGAT CATGATATTGATTATAAAGATGATGAT GATAAATGAAAGCTTGGCACTGGCCG Eurofins Genomics Restriction free JBD68 p-fimU 3xFLAG R: ATCACCATC ATGATCTTTATAATCTCCACCACTTCC ACCTGCGCAGCCAGAAGCGTCCCAAG Eurofins Genomics Restriction free (Continued on next page) Cell Reports 45, 116759, January 27, 2026 15
Recombinant:Article Title: A prophage-expressed type IV pilus component provides anti-phage defense.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse α-FLAG antibody (monoclonal) Sigma Cat# F3165; RRID: AB_259529 Goat α-mouse-AF555 Abcam Cat# AB150078; RRID:AB272519 Bacterial and virus strains P. aeruginosa PAO1 A. Davidson Lab GenBank: NC_002516.2 P. aeruginosa PAK A. Davidson Lab GenBank: LR657304.1 P. aeruginosa PAK (JBD68) A. Davidson Lab N/A P. aeruginosa PAK (LES2) A. Davidson Lab N/A P. aeruginosa PAK (LES3) A. Davidson Lab N/A P. aeruginosa PAK (JBD68 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAK (LES3 Δp-fimU) A. Davidson Lab N/A P. aeruginosa PAO1 ΔfimU A. Davidson Lab 16 N/A P. aeruginosa PAO1 ΔpilA A. Davidson Lab 42 N/A P. aeruginosa PAO1 ΔpilT A. Davidson Lab 42 N/A P. aeruginosa PAO1 I-C CRISPR A. Davidson Lab 43 N/A P. aeruginosa PAO1 I-C CRISPR Δhel A. Davidson Lab N/A P. aeruginosa PAO1 pilA A86C, pilT::Tn5 C. Ellison Lab 21 N/A E. coli DH5α A. Davidson Lab N/A E. coli SM10 λpir A. Davidson Lab N/A E. coli BTH101 A. Davidson Lab 32 N/A P. aeruginosa phage JBD68 A. Davidson Lab GenBank: KY707339.1 P. aeruginosa phage JBD68 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp4 A. Davidson Lab N/A P. aeruginosa phage JBD68 Δptp10 A. Davidson Lab N/A P. aeruginosa phage F10 A. Davidson Lab GenBank: NC_007805.1 P. aeruginosa phage LES2 A. Davidson Lab N/A P. aeruginosa phage LES3 A. Davidson Lab N/A P. aeruginosa phage LES3 Δp-fimU A. Davidson Lab N/A P. aeruginosa phage DMS3 A. Davidson Lab GenBank: NC_008717.1 P. aeruginosa phage D3112 A. Davidson Lab GenBank: AY394005.1 P. aeruginosa phage M6 A. Davidson Lab GenBank: NC_007809.1 P. aeruginosa phage F116 A. Davidson Lab GenBank: NC_006552.1 P. aeruginosa phage Luz19 A. Davidson Lab GenBank: NC_010326.1 P. aeruginosa phage AB05 A. Davidson Lab GenBank: NC_026602.1 P. aeruginosa phage ΦKMV A. Davidson Lab GenBank: NC_005045.1 P. aeruginosa phage ΦKMZ A. Davidson Lab N/A Chemicals, peptides, and recombinant proteins Tris Bioshop Cat# TRS003 Sodium chloride Bioshop Cat# SOD004 BioTryptone BioBasic Cat# TG217 Magnesium sulfate (heptahydrate) BioBasic Cat# M5BO329 L-(+)Arabinose Bioshop Cat# ARB222.100 Phosphate buffered saline Bioshop Cat# PBS408 Tween 20 Bioshop Cat# TWN508 (Continued on next page) Cell Reports 45, 116759, January 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Agar Bioshop Cat# AGR100.500 Ultra Pure Agarose Invitrogen Cat# 16500_500 Difco MacConkey Agar Base BD Cat# 281810 Difco Maltose BD Cat# 216830 X-Gal Biobasic Cat# BB0083 Mitomycin C Cell Signaling Technologies Cat# 51854S NcoI-HF New England Biolabs Cat# R3193S EcoRI-HF New England Biolabs Cat# R3101S HindIII-HF New England Biolabs Cat# R3104S KpnI-HF New England Biolabs Cat# R3142S XbaI-HF New England Biolabs Cat# R0145S BsaI-HF v2 New England Biolabs Cat# R3733S T4 DNA Ligase New England Biolabs Cat# M0202S T4 Polynucleotide Kinase New England Biolabs Cat# M0201S T4 DNA Ligase Buffer New England Biolabs Cat# B0202A DpnI ThermoFisher Cat# ER1705 Uranyl acetate Electron Microscopy Sciences Cat# 22400 Gentamycin sulfate Bioshop Cat# GTA401 Ampicillin Bioshop Cat# AMP201 Kanamycin Bioshop Cat# KAN201 AF488-maleimide ThermoFisher Cat# A10254 Crystal violet Bioshop Cat# CRY422.25 Carbon-coated TEM grids Electron Microscopy Sciences Cat# CF150-Cu Deposited data PAO1 and PAO1 (JBD68) RNA-Seq raw reads This study SRA: PRJNA1364051 Recombinant DNA pHERD30T Qiu et al. 44 N/A pEX18Gm Hmelo et al. 45 N/A pKT25 Karimova et al. 46 N/A pUT18C Karimova et al. 46 N/A PAO1 FimU This study N/A PA14 FimU This study N/A JBD68 P-FimU This study N/A Les P-FimU This study N/A F10 P-FimU This study N/A BWH P-FimU This study N/A CAAD P-FimU This study N/A NSYG P-FimU This study N/A Zip Taylor et al. 9 N/A LES3 Repressor This study N/A JBD68 P-FimU 3xFLAG This study N/A PAO1 FimU 3xFLAG This study N/A pPTP4 JBD68 This study N/A pPTP4 LES2 This study N/A T25 JBD68 P-FimU This study N/A T25 F10 P-FimU This study N/A T25 PAO1 FimU This study N/A T25 PAO1 FimS This study N/A (Continued on next page) 14 Cell Reports 45, 116759, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER T18 PAO1 FimS This study N/A T18 PAO1 PilA This study N/A T18 PAO1 PilE Marko et al. 31 N/A T18 PAO1 PilX Marko et al. 31 N/A T18 PAO1 PilV Marko et al. 31 N/A T18 PAO1 PilW Marko et al. 31 N/A T18 PAO1 PilU Marko et al. 31 N/A T18 PAO1 PilB Marko et al. 31 N/A Oligonucleotides PAO1 fimU F: AAACCATGGCCTC ATATCGTTCCAACTCGACCGG Eurofins Genomics NcoI PAO1 fimU R: AAAAAGCTTTCAA TAGCATGACTGGGGCGC Eurofins Genomics HindIII PA14 fimU F: AAACCATGGCCC GCTCTATTTGTCGCAGCGCC Eurofins Genomics NcoI PA14 fimU R: AAAAAGCTTTCA GTTACAGCTGTCCGGTTGCTTTG Eurofins Genomics HindIII JBD68 p-fimU F: AAACCATGGCC TACTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI JBD68 p-fimU R: AAAAAGCTTTC AGCAGCCAGAAGCGTCCC Eurofins Genomics HindIII F10 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTA Eurofins Genomics NcoI F10 p-fimU R: AAAAAGCTTCTAAC AGTCAAAAGTATCCCAGGATTCC Eurofins Genomics HindIII LES3 p-fimU F: AAACCATGGCCTA CTCTAGGTCGCGCGGATTTACC Eurofins Genomics NcoI LES3 p-fimU R: AAAAAGCTTTCAG CAGCCAGAAGTGTCCCA Eurofins Genomics HindIII BWH p-fimU F: AAACCATGGCC GGCTCCAAG Eurofins Genomics NcoI BWH p-fimU R: AAAAAGCTTCTAA CTGGGTTTTACAGCGCAGGc Eurofins Genomics HindIII CAAD p-fimU F: AAACCATGGGTT GAAATGTACTCTAGGTCGCGCG Eurofins Genomics NcoI CAAD p-fimU R: AAAAAGCTTTCAG GTTGGTTTCTTGGCGCA Eurofins Genomics HindIII NSYG p-fimU F: AAACCATGGATTC GCATAAATCAAGCTCAGCGTGG Eurofins Genomics NcoI NSYG p-fimU R: AAAAAGCTTCTAT TGGTCCTTGGCACACTGTG Eurofins Genomics HindIII LES3 repressor F: AAACCATGGCCA TCGATATCACGACAATCCGCCGCGCGAAC Eurofins Genomics NcoI LES3 repressor R: AAAAAGCTTTCAG TGTCGCCGCCGGCGCA Eurofins Genomics HindIII JBD68 p-fimU 3xFLAG F: TATAAAGAT CATGATATTGATTATAAAGATGATGAT GATAAATGAAAGCTTGGCACTGGCCG Eurofins Genomics Restriction free JBD68 p-fimU 3xFLAG R: ATCACCATC ATGATCTTTATAATCTCCACCACTTCC ACCTGCGCAGCCAGAAGCGTCCCAAG Eurofins Genomics Restriction free (Continued on next page) Cell Reports 45, 116759, January 27, 2026 15
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